Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transducer Mechanism: Enzyme-Linked Receptors

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

The EMBO journal·2026

Same author

Paracrine signals from HIV-1-infected immune cells reprogram cervical cancer pathways.

iScience·2026

Same author

Corona: A Virtual Mass Spectrometer for the Development of Real-Time Mass Spectrometry Software.

Analytical chemistry·2026

Same author

A multi-omics study reveals pathway-level insights and predictive biomarkers in pediatric TB.

Clinical proteomics·2026

Same author

Coronavirus protein interaction mapping in bat and human cells reveals network rewiring governing immune evasion and zoonotic potential.

Cell host & microbe·2026

Same author

MEK interactions tune RAF kinase sensitivity to conformation-selective inhibition.

Nature chemical biology·2026

Search research articles

Related Experiment Video

Updated: May 17, 2026

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

Affinity-based probes based on type II kinase inhibitors.

Pratistha Ranjitkar¹, B Gayani K Perera, Danielle L Swaney

¹Department of Chemistry, University of Washington, Seattle, Washington 98195, USA.

Journal of the American Chemical Society

|October 24, 2012

Summary

New probes profile protein kinases in complex biological samples. This chemical proteomics approach identifies active kinases in cell extracts and live cells for therapeutic target discovery.

More Related Videos

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Published on: January 22, 2019

Related Experiment Videos

Last Updated: May 17, 2026

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Published on: January 22, 2019

Area of Science:

Biochemistry
Chemical Biology
Proteomics

Background:

Protein kinases are crucial in mammalian signal transduction and represent important therapeutic targets.
Studying protein kinase function necessitates advanced techniques for profiling within complex proteomes.
Existing methods may lack the specificity or applicability for profiling kinases in native cellular environments.

Purpose of the Study:

To develop and validate novel active site-directed probes for profiling protein kinases.
To enable the characterization of kinase activity in both cell extracts and live cells.
To advance chemical proteomics for interrogating kinase active sites in physiological contexts.

Main Methods:

Design of probes featuring general ligands to stabilize inactive kinase conformations.
Incorporation of trifluoromethylphenyl diazirine for covalent modification.
Utilizing alkyne moieties for efficient kinase enrichment and identification.
Application of the probes in whole cell extracts and live mammalian cells.

Main Results:

Successful profiling of diverse serine/threonine and tyrosine kinases in whole cell extracts.
Selective labeling and identification of kinase targets within live cells.
Demonstration of the probes' ability to capture kinases in specific inactive conformations.
Validation of a chemical proteomics strategy for kinase active site interrogation.

Conclusions:

The developed active site-directed probes offer a powerful tool for kinase profiling.
This chemical proteomics approach is effective in complex biological systems, including live cells.
The technology provides valuable insights into kinase activity in physiologically relevant environments.
These probes can aid in the discovery and validation of therapeutic kinase targets.