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Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Published on: November 26, 2018

BRAF mutations in chronic lymphocytic leukemia.

Billy Michael Chelliah Jebaraj¹, Dirk Kienle, Andreas Bühler

¹Department of Internal Medicine III, University of Ulm, Ulm, Germany.

Leukemia & Lymphoma

|October 24, 2012

Summary

BRAF mutations are rare in chronic lymphocytic leukemia (CLL) and B-cell prolymphocytic leukemia (B-PLL). BRAF inhibition did not impact cell viability in CLL samples, suggesting limited therapeutic relevance.

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Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation

Published on: August 23, 2024

Area of Science:

Hematology
Oncology
Molecular Biology

Background:

BRAF mutations are frequent in melanoma and thyroid cancer.
Their role in hematological malignancies, including chronic lymphocytic leukemia (CLL), is less understood.

Purpose of the Study:

To investigate the incidence of BRAF mutations in CLL and B-cell prolymphocytic leukemia (B-PLL).
To assess if BRAF mutations are selected during disease progression.
To evaluate the effect of BRAF inhibition on CLL cell viability.

Main Methods:

Sequencing of BRAF exons 11 and 15 in 138 CLL and 32 B-PLL cases.
Analysis of BRAF mutation incidence in a fludarabine-refractory CLL cohort.
In vitro assessment of BRAF inhibitor PLX4720 on primary CLL cells.

Main Results:

BRAF mutations were found in 2.8% of CLL cases; none were detected in B-PLL.
No increased mutation incidence was observed in fludarabine-refractory CLL.
BRAF inhibition did not induce cell death in CLL cells, irrespective of mutation status.

Conclusions:

BRAF mutations occur infrequently in CLL.
BRAF mutations do not appear to be selected during CLL progression.
Targeting BRAF may have limited therapeutic potential in CLL.