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Automated isolation of mononuclear cells using the Fenwal CS3000 blood cell separator
E M Areman1, H Cullis, R A Sacher
1Division of Transfusion Medicine and Bone Marrow Transplantation, Georgetown University Hospital, Washington, D.C.
Progress in Clinical and Biological Research
|January 1, 1990
Abstract:
We describe a method for in vitro isolation of mononuclear cells from peripheral blood or bone marrow using a Fenwal CS3000 Apheresis device without employing density gradients or sedimenting agents. The automatic processing program requires minimal operator intervention and no subjective operator decisions. A mean of 67% of starting mononuclear cells were recovered in a 100 ml product having 95% mononuclear cells and less than 1% of the original red blood cells. The average processing time was 35 minutes.