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Related Experiment Video

Updated: May 17, 2026

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Efflux: how bacteria use pumps to control their microenvironment.

E David G McIntosh¹

¹Faculty of Medicine, Imperial College, London, UK. e.mcintosh@imperial.ac.uk

Handbook of Experimental Pharmacology

|October 24, 2012

Summary

Efflux pumps drive antibiotic resistance. Targeting these pumps with new drugs, like modified tetracyclines (tigecycline), can restore antibiotic effectiveness against resistant bacteria.

Area of Science:

Microbiology
Pharmacology
Infectious Diseases

Background:

Efflux pumps are a major mechanism contributing to antimicrobial resistance.
These pumps actively expel antibiotics from bacterial cells, reducing drug efficacy.
Overcoming efflux-mediated resistance is crucial for effective treatment of bacterial infections.

Purpose of the Study:

To explore efflux pumps as therapeutic targets for antimicrobial drug development.
To discuss strategies for designing new antibacterial agents that overcome efflux.
To highlight the potential of modifying existing antibiotic classes to combat efflux.

Main Methods:

Review of current literature on efflux pump mechanisms and their role in resistance.
Analysis of case studies, such as tigecycline, demonstrating successful modification of antibiotics.

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In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

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Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Published on: December 3, 2010

Related Experiment Videos

Last Updated: May 17, 2026

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

Published on: February 17, 2014

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Published on: December 3, 2010

Discussion of drug design principles for targeting efflux pumps.

Main Results:

Efflux pumps represent a significant challenge in treating bacterial infections.
Modifying existing antibiotic scaffolds, like tetracyclines, can resensitize bacteria to drugs.
Tigecycline serves as a successful example of overcoming efflux-mediated resistance.

Conclusions:

Targeting bacterial efflux pumps is a viable strategy for developing novel antimicrobial therapies.
Repurposing and modifying existing antibiotics can be an effective approach to combat resistance.
Further research into efflux pump inhibitors and efflux-resistant antibiotics is warranted.