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Updated: May 17, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
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A Quantitative Glycomics and Proteomics Combined Purification Strategy

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Solid-phase glycan isolation for glycomics analysis.

Shuang Yang1, Hui Zhang

  • 1Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD 21231, USA.

Proteomics. Clinical Applications
|October 24, 2012
PubMed
Summary
This summary is machine-generated.

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Glycosylation, a key protein modification, impacts protein function and disease. Advanced glycan analysis using HPLC-MS and improved isolation techniques are crucial for understanding these roles in complex biological samples.

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Glycomics

Background:

  • Glycosylation is a critical post-translational modification (PTM) significantly altering protein biological activities.
  • Understanding glycan structures in glycoproteins is vital for correlating glycan function with disease mechanisms.
  • High-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) is a standard for glycan profiling.

Purpose of the Study:

  • To highlight the importance of glycan structural analysis in complex biological and clinical samples.
  • To review methods for glycan isolation and purification essential for accurate mass spectrometry-based analysis.
  • To discuss advancements in glycan isolation techniques for improved specificity and yield in glycomics.

Main Methods:

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Last Updated: May 17, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Published on: January 18, 2020

  • Glycan isolation and purification methods including solid-phase extraction (SPE), liquid-phase extraction, chromatography, and electrophoresis.
  • Traditional affinity-based isolation using hydrophobic and hydrophilic columns, followed by hydrophilic interaction LC on graphite-activated carbon.
  • Development of specific chemical methods (hydrazide or oxime) for solid-phase glycan isolation.

Main Results:

  • Established methods for glycan isolation are critical to remove salts and interfering ions that hinder MS ionization.
  • Traditional methods can suffer from non-specific binding, impacting glycan purity and quantification.
  • Chemical methods offer high specificity and yield for glycan isolation, overcoming limitations of affinity-based approaches.

Conclusions:

  • Accurate glycan profiling requires effective isolation and purification strategies to ensure reliable MS data.
  • Specific glycan isolation techniques, particularly chemical methods, are essential for advancing glycomics research.
  • Combined with high-resolution MS, these advanced isolation methods offer powerful tools for comprehensive glycomics analysis.