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Detection and quantitative determination of 3-nitropropionic acid in bovine urine
1Research Station, Agriculture Canada, Kamloops, BC.
Toxicology Letters
|February 1, 1990
Abstract:
The separation and determination of 3-nitropropionic acid (NPA) in bovine urine by reverse-phase HPLC is described. The method utilizes a rapid ethyl acetate extraction and this clean-up step also improves the chromatographic resolution of NPA in bovine plasma. A simple colorimetric method is also developed for the rapid detection and estimation of NPA in urine and the data indicate that it is in good agreement with the HPLC procedure.