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Updated: May 17, 2026

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs
Published on: February 2, 2024
Discovery of novel integrin ligands from combinatorial libraries using a multiplex "beads on a bead" approach
Choi-Fong Cho1, Giulio A Amadei, Daniel Breadner
1Department of Medical Biophysics, MSB-415A, The University of Western Ontario, London, Ontario, N6A 5C1 Canada.
Abstract:
The development of screening approaches to identify novel affinity ligands has paved the way for a new generation of molecular targeted nanomedicines. Conventional methods typically bias the display of the target protein to ligands during the screening process. We have developed an unbiased multiplex "beads on a bead" strategy to isolate, characterize, and validate high affinity ligands from OBOC libraries. Novel non-RGD peptides that target α(v)β(3) integrin were discovered that do not affect cancer or endothelial cell biology. The peptides identified here represent novel integrin-targeted agents that can be used to develop targeted nanomedicines without the risk of increased tumor invasion and metastasis.
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