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IEF-based multidimensional applications in proteomics: toward higher resolution

Alexander Stoyanov1

  • 1Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO 65212, USA. stoyanova@health.missouri.edu

Electrophoresis
|October 26, 2012
PubMed

This review focuses on the alternative isoelectrofocusing methods and IEF-related techniques in protein analysis and characterization. The main emphasis is placed on the evaluation of the resolving power of new approaches, as well as potential advantages of IEF-related techniques for multidimensional analysis. In particular, the inverse 2D gel electrophoresis where the Mw separation stage precedes IEF mode is considered.

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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