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Measurement of Bioelectric Current with a Vibrating Probe
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Published on: January 4, 2011

CCVJ is not a simple rotor probe.

Christopher Rumble1, Kacie Rich, Gang He

  • 1Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.

The Journal of Physical Chemistry. A
|October 27, 2012
PubMed
Summary
This summary is machine-generated.

The rotor probe CCVJ

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Area of Science:

  • Photochemistry
  • Molecular probes
  • Fluorescence spectroscopy

Background:

  • The rotor probe 9-(2-carboxy-2-cyanovinyl)julolidine (CCVJ) exhibits apparent sensitivity to fluid motion.
  • This effect is not observed in the related molecule 9-(dicyanovinyl)julolidine (DCVJ).
  • The commonly assumed TICT process is thought to confer viscosity-sensing ability.

Purpose of the Study:

  • To investigate the photochemical properties of CCVJ.
  • To elucidate the mechanism behind its apparent sensitivity to fluid flow.
  • To clarify the role of photoisomerization versus TICT in its sensing capabilities.

Main Methods:

  • Photochemical studies of CCVJ in hydroxylic solvents.
  • Analysis of E and Z isomers in solution under varying light conditions.
  • Comparison of CCVJ's behavior with DCVJ.

Main Results:

  • CCVJ's viscosity-sensing ability is an indirect effect of photoisomerization, not TICT.
  • CCVJ exists as an E/Z photoisomeric mixture in solution.
  • The Z isomer is nonfluorescent and metastable, complicating fluorescence response.

Conclusions:

  • Photoisomerization, not TICT, is responsible for CCVJ's viscosity-sensing properties.
  • The formation of long-lived photoproducts in CCVJ complicates its use as a steady-state sensor.
  • Careful consideration of isomerization is needed when using CCVJ and similar probes.