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Updated: May 17, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Profiling the kinome: current capabilities and future challenges.

James D R Knight¹, Tony Pawson, Anne-Claude Gingras

¹Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.

Journal of Proteomics

|October 27, 2012

Summary

This summary is machine-generated.

Protein kinases are crucial in cell biology and medicine. This review highlights high-throughput methods for studying the kinome, enabling researchers to answer key questions about kinase activity and function.

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Area of Science:

Biochemistry
Molecular Biology
Cell Signaling

Background:

Protein kinases represent the second-largest human protein family.
They are central to numerous cellular processes and are a major focus of biological and medical research.
Understanding kinase function is critical for both basic science and therapeutic applications.

Purpose of the Study:

To review and outline promising high-throughput techniques for studying the human kinome.
To provide strategies for addressing key questions in kinase-centric research.
To bridge the gap between protein structure and clinical applications in kinase research.

Main Methods:

Discussion of various experimental and computational approaches for kinome analysis.
Focus on methods enabling high-throughput screening and analysis.
Integration of techniques for studying kinase activity, substrate targeting, and phosphorylation site identification.

Main Results:

Identification of diverse strategies to investigate kinase roles in specific cellular states and responses.
Elucidation of methods to assess the impact of modulating kinase activity.
Presentation of approaches to determine kinase-substrate relationships and identify specific phosphorylation events.

Conclusions:

The review provides a comprehensive overview of advanced, high-throughput methods for kinome research.
These strategies empower researchers to address complex questions regarding kinase function and regulation.
The discussed approaches facilitate the translation of fundamental kinase knowledge towards clinical applications.