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PPAR-alpha cloning, expression, and characterization.

Suong N T Ngo1, Ross A McKinnon

  • 1The University of Adelaide, Roseworthy, SA, Australia. suong.ngo@.adelaide.edu.au

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2012
PubMed
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Peroxisome proliferator-activated receptor α (PPARα) cloning methods are detailed, including RT-PCR and RACE. These techniques enable the study of PPARα gene expression in mammalian cells, crucial for understanding metabolic regulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Peroxisome proliferator-activated receptor α (PPARα) is a nuclear receptor regulating metabolic genes.
  • PPARα isoforms (β, δ, γ) are part of the steroid receptor superfamily.
  • PPARα exhibits tissue- and species-specific expression across various organisms.

Purpose of the Study:

  • To describe methods for cloning PPARα genes.
  • To present protocols for studying cloned PPARα cDNA expression in mammalian cells.

Main Methods:

  • Cloning of PPARα genes using Reverse Transcription Polymerase Chain Reaction (RT-PCR).
  • Cloning of PPARα genes using Rapid Amplification of cDNA Ends (RACE).
  • Studying cloned PPARα cDNA expression in mammalian cell systems.

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Last Updated: May 17, 2026

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Main Results:

  • Established RT-PCR and RACE protocols for PPARα gene cloning.
  • Developed methods for analyzing PPARα gene expression in mammalian cells.

Conclusions:

  • The described methods facilitate the cloning and expression analysis of PPARα.
  • This research aids in understanding the role of PPARα in metabolic regulation across species.