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Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
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Cellulolytic enzymes on lignocellulosic substrates in solid state fermentation by Aspergillus niger.

M Subhosh Chandra1, Buddolla Viswanath, B Rajasekhar Reddy

  • 1Department of Microbiology, Sri Krishnadevaraya University, Anantapur, 515 003 India.

Indian Journal of Microbiology
|October 27, 2012
PubMed
Summary

Wheat bran and groundnut fodder are effective substrates for Aspergillus niger to produce cellulolytic enzymes via solid-state fermentation. Peak enzyme production occurred within three days, highlighting their potential for industrial applications.

Keywords:
Aspergillus nigerCellulolytic enzymesGroundnut fodderLignocellulosic substratesRice branSawdustSolid state fermentationWheat bran

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Area of Science:

  • Biotechnology
  • Enzyme Technology
  • Microbial Fermentation

Background:

  • Cellulolytic enzymes are crucial for breaking down lignocellulosic biomass.
  • Aspergillus niger is a well-known microorganism for enzyme production.
  • Solid-state fermentation (SSF) offers advantages for enzyme production using agro-industrial wastes.

Purpose of the Study:

  • To compare the efficacy of different lignocellulosic substrates for cellulolytic enzyme production by Aspergillus niger using SSF.
  • To determine the optimal substrate and fermentation time for maximum enzyme yield.

Main Methods:

  • Aspergillus niger was cultivated on groundnut fodder, wheat bran, rice bran, and sawdust in SSF.
  • Czapek Dox broth with 0.5% cellulose was used for moistening the substrates.
  • Production of filter paperase (FPase), carboxymethyl cellulase (CMCase), and β-glucosidase was monitored over 5 days.

Main Results:

  • Wheat bran and groundnut fodder were superior substrates for cellulolytic enzyme production compared to rice bran and sawdust.
  • Peak enzyme production for FPase, CMCase, and β-glucosidase was observed within 3 days of incubation.
  • Groundnut fodder yielded significant FPase (2.09 FPU/g), CMCase (1.36 U/g), and β-glucosidase (0.0117 U/g).
  • Maximum protein secretion (5.10 mg/g) was recorded on groundnut fodder on day 1.

Conclusions:

  • Wheat bran and groundnut fodder are highly suitable substrates for Aspergillus niger in SSF for cellulolytic enzyme production.
  • The study demonstrates the potential of utilizing agricultural byproducts for cost-effective enzyme manufacturing.