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Updated: May 17, 2026

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Simultaneous detection of major enteric viruses using a combimatrix microarray.

Ju-Mi Kim¹, Sung Yeon Kim, Young Bin Park

¹Department of Environmental Health and Institute of Health and Environment, School of Public Health, Seoul National University, Seoul 151-742, Republic of Korea.

Journal of Microbiology (Seoul, Korea)

|October 30, 2012

Summary

A new multiplex RT-PCR and microarray assay enables sensitive, simultaneous detection of major enteric viruses like norovirus and rotavirus. This high-throughput method aids in diagnosing viral gastroenteritis in clinical and environmental samples.

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Published on: November 15, 2017

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Last Updated: May 17, 2026

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

Virology
Molecular Biology
Public Health

Background:

Enteric viruses (norovirus, rotavirus, adenovirus, astrovirus) are leading causes of food- and water-borne disease outbreaks and gastroenteritis globally.
Sensitive, high-throughput detection methods are crucial for accurate diagnosis and public health improvement.

Purpose of the Study:

To develop a sensitive, specific, and high-throughput analytical assay for the simultaneous detection of major enteric viral pathogens.
To utilize the Combimatrix platform with oligonucleotide probes for enhanced viral detection.

Main Methods:

Developed a multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay targeting partial gene sequences of four enteric viruses.
Employed fluorescent labeling for subsequent microarray analysis.
Designed and selected specific oligonucleotide probes for each target virus on the microarray.

Main Results:

The multiplex RT-PCR assay successfully amplified partial gene sequences of the four targeted enteric viruses.
The oligonucleotide microarray demonstrated sensitive and simultaneous detection of these viruses in single and mixed infections.
The assay showed high specificity in detecting the targeted viral pathogens.

Conclusions:

The developed multiplex RT-PCR and microarray assay is a sensitive, specific, and high-throughput tool for detecting major enteric viruses.
This method is valuable for diagnosing enteric viral infections in both clinical and environmental specimens.
The assay contributes to improved public health surveillance and outbreak response for viral gastroenteritis.