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Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research
Published on: April 19, 2017
Protein antigen delivery by gene gun-mediated epidermal antigen incorporation (EAI)
Sandra Scheiblhofer1, Uwe Ritter, Josef Thalhamer
1Division of Allergy and Immunology, Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
Abstract:
The gene gun technology can not only be employed for efficient transfer of gene vaccines into upper layers of the skin, but also for application of protein antigens. As a tissue rich in professional antigen presenting cells, the skin represents an attractive target for immunizations. In this chapter we present a method for delivery of the model antigen ovalbumin into the skin of mice termed epidermal antigen incorporation and describe in detail how antigen-specific proliferation in draining lymph nodes can be followed by flow cytometry.
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