Modified assay procedure for the estimation of serum glucose using microwell reader

Area of Science:

• Biochemistry
• Clinical Chemistry
• Analytical Chemistry

Background:

• Accurate blood glucose level determination is crucial for assessing human physiological condition and identifying abnormalities in glucose metabolism due to hormonal imbalances.
• Traditional colorimetric and titrimetric methods for glucose estimation are often time-consuming and expensive.
• There is a need for more efficient and cost-effective methods for blood glucose monitoring.

Purpose of the Study:

• To develop and validate a modified colorimetric microwell reader method for blood glucose estimation.
• To compare the efficiency, cost, and linearity of the modified method with traditional colorimetric analysis.
• To assess the suitability of the modified method for high-throughput sample screening.

Main Methods:

• A modified colorimetric microwell reader method was developed for blood glucose determination.
• The method utilized small quantities of samples and reagents.
• Assay performance was evaluated based on linearity and compared to conventional colorimetric techniques.

Main Results:

• The modified colorimetric microwell reader method demonstrated comparable linearity to standard colorimetric analysis.
• The cost of glucose estimation using the modified method was reduced to approximately one-third of the traditional method.
• The modified method allows for the screening of a large number of samples in a significantly shorter time.

Conclusions:

• The modified colorimetric microwell reader method provides a cost-effective and time-efficient alternative for blood glucose level determination.
• This optimized method is suitable for rapid screening of large sample volumes.
• The technique supports accurate physiological condition assessment through efficient glucose metabolism monitoring.