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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Eleven diverse nuclear-encoded phylogenetic markers for the subfamily Panicoideae (Poaceae)
Matt C Estep1, Dilys M Vela Diaz, Jinshun Zhong
1Department of Biology, University of Missouri-St. Louis, 8001 Natural Bridge Road, St. Louis, Missouri 63121 USA. mcestep@gmail.com
Premise Of The Study:
Polyploidy is common in the grasses and low-copy nuclear loci are needed to further our understanding of phylogenetic relationships.
Methods And Results:
Genetic and genomic resources were combined to identify loci known to influence plant and inflorescence architecture. Degenerate primers were designed and tested to amplify regions of 11 nuclear-encoded loci across the panicoid grasses.
Conclusions:
The primers designed in this study amplify regions of a diverse set of genes within the panicoid grasses. Properly employed, these markers will allow the identification of allopolyploid taxa and their diploid progenitors.
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