Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 17, 2026

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
07:09

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Microscale methods to assemble mammalian cells into tissue-like structures.

Peiyuan Gong1, Wen Zheng, Dan Xiao

  • 1College of Chemistry, Sichuan University, Cheng 610064, China.

Science China. Life Sciences
|October 31, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-Lasting Chemiluminescence Based on Functionalized Multicolor Protein Capsules for Multiple Visualization Detection of Avian Influenza Virus Biomarkers.

Analytical chemistry·2024
Same author

Spatio-temporal mapping of leaf area index in rice: spectral indices and multi-scale texture comparison derived from different sensors.

Frontiers in plant science·2024
Same author

Influence of autonomic nervous dysfunction on eating during hemodialysis sessions: An observational study.

Medicine·2024
Same author

A high-safety lithium-ion battery electrospun separator with Si<sub>3</sub>N<sub>4</sub>-assisted sulfonated poly(ether ether ketone) for regulating lithium flux.

Journal of colloid and interface science·2024
Same author

Intelligent Analysis of Avian Influenza Virus Biomarkers Based on Chemiluminescence Resonance Energy Transfer and DNA Logic Analysis.

ACS applied materials & interfaces·2024
Same author

Blue emission-dominated NaYbF<sub>4</sub>@NaYF<sub>4</sub>:2%Ho@NaYF<sub>4</sub> upconversion nanoparticles for detecting ascorbic acid.

Nanoscale·2024

This review explores microscale methods for assembling mammalian cells into 3D tissue structures. These bottom-up approaches are crucial for advancing cell biology research and tissue engineering (TE).

Area of Science:

  • Cell Biology
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Tissues and organs are hierarchically organized, with cells communicating in a complex 3D environment.
  • In vitro recapitulation of tissue-like structures is vital for fundamental research and tissue engineering (TE).
  • Current TE employs top-down (scaffold-based) and bottom-up (building block assembly) strategies.

Purpose of the Study:

  • To review microscale methods for controlling mammalian cell assembly into 3D tissue-like structures.
  • To highlight the advantages of the bottom-up approach in TE.

Main Methods:

  • Focus on microscale techniques for precise cell control and organization.
  • Exploration of physical and chemical methods for creating tissue building blocks.

More Related Videos

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Related Experiment Videos

Last Updated: May 17, 2026

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
07:09

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

  • Leveraging repeating structural and functional units for cell-cell interactions.

    • Main Results:

    • Overview of available microscale methods for bottom-up tissue construction.
    • Demonstration of how these methods facilitate the creation of functional cellular units.
    • Emphasis on precision-engineered microscale features for controlled assembly.

    Conclusions:

    • Microscale methods offer powerful tools for bottom-up tissue engineering.
    • These approaches enable the creation of complex 3D tissue-like structures from mammalian cells.
    • Advancements in microscale control are key to future tissue engineering innovations.