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Related Experiment Video

Updated: May 17, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

DNA sequencing and bar-coding using solid-state nanopores.

Evrim Atas¹, Alon Singer, Amit Meller

¹Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.

Electrophoresis

|October 31, 2012

Summary

This summary is machine-generated.

Nanopore sensors offer advanced single-molecule DNA sequencing and genotyping. These methods use optical detection and biochemical conversions for high-throughput analysis and precise gene identification, including viral subtypes.

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Published on: October 31, 2013

Area of Science:

Nanotechnology
Molecular Biology
Genomics

Background:

Nanopores are powerful tools for single-molecule analysis.
Genomic applications are a key area of promise for nanopore technology.

Purpose of the Study:

To review two applications of nanopore sensors: DNA sequencing and single-molecule genotyping.
To present a nanopore-based DNA sequencing method with optical detection for high throughput.
To describe nanopore use for single-molecule genotyping of similar genes.

Main Methods:

DNA sequencing employs biochemical DNA expansion, converting nucleobases to unique 16-mers.
Converted DNA strands hybridize to fluorophore-tagged molecular beacons for optical detection via nanopores.
Genotyping uses peptide nucleic acid probes and electronic characterization of complexes within solid-state nanopores.

Main Results:

Optical detection of photon bursts allows for sequencing by sequentially removing molecular beacons.
Nanopore genotyping can differentiate genes with high sequence similarity at the single-molecule level.
Demonstrated differentiation of pol genes from two similar HIV subtypes.

Conclusions:

Nanopore technology enables advanced DNA sequencing with massively parallel throughput.
Single-molecule genotyping using nanopores offers high precision for differentiating similar genetic sequences.
This technology presents a novel diagnostics platform for viral classification and personalized medicine.