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Updated: May 17, 2026

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets
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CLEIA CA125 evidences: good analytical performance avoiding "Hook effect".

R Falzarano1, V Viggiani, S Michienzi

  • 1Department of Molecular Medicine, "Sapienza" University, Viale Regina Elena 324, 00161, Rome, Italy.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|November 1, 2012
PubMed
Summary
This summary is machine-generated.

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This study compared radioimmunometric assay (RIA) and automated chemiluminescent enzyme immunoassay (CLEIA) for Cancer Antigen 125 (CA125) testing. CLEIA demonstrated good reliability, suggesting it is preferable to RIA for minimizing errors in CA125 measurements.

Area of Science:

  • Biochemistry
  • Oncology
  • Clinical Chemistry

Background:

  • Cancer antigen 125 (CA125) is a key glycoprotein marker for ovarian cancer monitoring.
  • Accurate CA125 measurement is crucial for tracking disease progression and treatment response.
  • Evaluating assay reliability is essential for clinical decision-making.

Purpose of the Study:

  • To assess the reliability of radioimmunometric assay (RIA) and automated chemiluminescent enzyme immunoassay (CLEIA) for CA125.
  • To compare CA125 serum levels measured by RIA and CLEIA in a diverse patient cohort.
  • To identify potential discrepancies and artifacts between the two assay methods.

Main Methods:

  • Collected serum samples from 357 participants (86 healthy donors, 271 oncologic patients).

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  • Measured CA125 levels using both RIA and CLEIA systems.
  • Analyzed results for agreement, specifically noting differences in oncologic patient samples and investigating RIA 'hook effect' artifacts.

    • Main Results:

    • High agreement (R²=0.9003) observed between RIA and CLEIA in healthy donors.
    • Significant differences detected in nine oncologic patient samples between the two assays.
    • Dilution of RIA samples resolved discrepancies, indicating a 'hook effect' in RIA and high agreement (R²=0.9847) with undiluted CLEIA values.

    Conclusions:

    • Both RIA and CLEIA show overall good comparison for CA125 measurement.
    • RIA may exhibit artifacts like the 'hook effect', potentially leading to errors.
    • Automated CLEIA offers superior reliability and is recommended over one-step RIA for CA125 analysis.