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Related Experiment Video

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Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
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Improved alignment of reticulocyte counts between Sysmex XT-2000i instruments.

Michael Ashenden1, Ken Sharpe, James Habel

  • 1SIAB Research, Gold Coast, Queensland, Australia. heyasho@hotmail.com

Journal of Clinical Pathology
|November 1, 2012
PubMed
Summary

Recalibrating automated haematology analysers like the Sysmex XT-2000i improves the comparability of reticulocyte counts. This is crucial for detecting blood doping in athletes using longitudinal data from different labs.

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Area of Science:

  • Clinical Chemistry
  • Haematology
  • Sports Science

Background:

  • Reticulocyte counts are key for detecting blood doping in athletes.
  • Comparability of reticulocyte counts is vital due to longitudinal data from diverse laboratories.

Purpose of the Study:

  • To improve the between-instrument comparability of reticulocyte counts.
  • Focus on the Sysmex XT-2000i automated haematology analyser.

Main Methods:

  • Optimized recalibration of instruments using control material (e-CHECK).
  • Verification performed with fresh blood samples.
  • Ensured comparability across multiple Sysmex XT-2000i instruments.

Main Results:

  • Recalibration achieved mean reticulocyte count differences within 0.1% between instruments.
  • Tested on three Sysmex XT-2000i analysers.
  • Results based on reticulocyte percentage of total cells in fresh blood.

Conclusions:

  • A straightforward method to reduce between-instrument variability in reticulocyte counts.
  • Enhances reliability of reticulocyte data for anti-doping efforts.
  • Applicable to Sysmex XT-2000i haematology analysers.