Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 17, 2026

Bioluminescent Bacterial Imaging In Vivo
05:06

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Bioluminescent capsules for live-cell imaging.

Sung Bae Kim1, Yuzuru Ito, Masaki Torimura

  • 1Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki 305-8569, Japan. kimu-sb@aist.go.jp

Bioconjugate Chemistry
|November 2, 2012
PubMed
Summary

Researchers developed a bioluminescent capsule to track cell signaling and protein localization. This new tool enhances bioluminescence for imaging cellular processes in living cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Assessment of Capillary Permeability in Deep Intracardiac Capillaries Using Fluorescent Dextran.

Bio-protocol·2026
Same author

Curative Surgery for Malignant Sigmoid-Duodenal Fistula Caused by Colon Cancer: A Case Report.

Surgical case reports·2026
Same author

Erratum to "Assessment of permeability in deep tissue capillaries using a new method reflects the nutrient supply status in a healthy heart" [Regen Ther, 30C (2025), 1019].

Regenerative therapy·2025
Same author

Assessment of permeability in deep tissue capillaries using a new method reflects the nutrient supply status in a healthy heart.

Regenerative therapy·2025
Same author

Considerations for developing CYP induction assays in hepatocytes: Insights from a multilaboratory study.

European journal of cell biology·2025
Same author

[Microphysiological System(MPS)for Drug Research, the Current Situation and the Future].

Gan to kagaku ryoho. Cancer & chemotherapy·2025

Area of Science:

  • Biotechnology
  • Cell Biology
  • Molecular Imaging

Background:

  • Cell signaling and protein localization are crucial for understanding cellular functions.
  • Existing methods for imaging these processes can be limited in sensitivity and duration.
  • Bioluminescence offers a sensitive detection method but requires efficient delivery and activation strategies.

Purpose of the Study:

  • To design and validate a novel bioluminescent capsule for illuminating cell signaling and protein localization.
  • To enhance bioluminescence intensity and duration by targeting the capsule to the plasma membrane.
  • To demonstrate the capsule's utility in imaging specific cellular events, such as apoptosis.

Main Methods:

  • A bioluminescent capsule was engineered with four components: secretion peptide (SP), luciferase body, cargo protein, and membrane-localization signal (MLS).

More Related Videos

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
09:00

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
07:28

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Published on: November 24, 2017

Related Experiment Videos

Last Updated: May 17, 2026

Bioluminescent Bacterial Imaging In Vivo
05:06

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
09:00

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
07:28

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Published on: November 24, 2017

  • The capsule was designed for targeted delivery to the plasma membrane (PM) and triggered release of luciferase upon external signals.
  • Staurosporine (STS)-activated apoptosis signaling was imaged using a capsule carrying a DEVD peptide.

    • Main Results:

    • The plasma membrane localization strategy significantly prolonged and strengthened bioluminescence intensity.
    • The capsule efficiently imaged staurosporine-induced apoptosis signaling.
    • Functional proteins, including fluorescent proteins and luciferases, were effectively transported to the membrane.
    • A 60-nm red-shifted bioluminescence was observed when using fused luciferases or fluorescent proteins in living cells.

    Conclusions:

    • The developed bioluminescent capsule provides a novel strategy for illuminating cellular signals in living mammalian cells.
    • Targeting the capsule to the plasma membrane enhances signal detection and duration.
    • This system offers a versatile platform for studying various cellular processes and protein dynamics.