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Updated: May 17, 2026

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Published on: September 20, 2016
Early evolution of efficient enzymes and genome organization
András Szilágyi1, Adám Kun, Eörs Szathmáry
1Department of Plant Taxonomy and Ecology, Institute of Biology, Eötvös University, Pázmány Péter sétány 1/C, 1117, Budapest, Hungary.
Background:
Cellular life with complex metabolism probably evolved during the reign of RNA, when it served as both information carrier and enzyme. Jensen proposed that enzymes of primordial cells possessed broad specificities: they were generalist. When and under what conditions could primordial metabolism run by generalist enzymes evolve to contemporary-type metabolism run by specific enzymes?
Results:
Here we show by numerical simulation of an enzyme-catalyzed reaction chain that specialist enzymes spread after the invention of the chromosome because protocells harbouring unlinked genes maintain largely non-specific enzymes to reduce their assortment load. When genes are linked on chromosomes, high enzyme specificity evolves because it increases biomass production, also by reducing taxation by side reactions.
Conclusion:
The constitution of the genetic system has a profound influence on the limits of metabolic efficiency. The major evolutionary transition to chromosomes is thus proven to be a prerequisite for a complex metabolism. Furthermore, the appearance of specific enzymes opens the door for the evolution of their regulation.
Reviewers:
This article was reviewed by Sándor Pongor, Gáspár Jékely, and Rob Knight.
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