[Genetic polymorphism of human platelet antigens 1-18 in Chinese Nanjing Han population]

The purpose of this study was to investigate the allele frequencies of the human platelet alloantigens 1-18 system (HPA-1-18) in Chinese Nanjing unrelated and healthy Han population, so as to provide the credible basis to screen compatible platelets for transfusing patients. The genotypes of 18 HPA systems were determined by polymerase chain reaction using sequence-specific primer (PCR-SSP) for 300 samples. The results showed that the gene frequencies obtained from 300 Nanjing unrelated population were 0.9183 and 0.0817 for HPA-2a and -2b, 0.6100 and 0.3900 for HPA-3a and -3b, 0.9733 and 0.0267 for HPA-5a and -5b, 0.9883 and 0.0117 for HPA-6a and -6b, 0.5250 and 0.4750 for HPA-15a and -15b. All the tested individuals were homozygotes for HPA-1a, -4a, -7a-14a and HPA -16a-18a. There was a good fit to Hardy-Weinberg equilibrium in each group. It is concluded that this study has confirmed the ethnic and regional difference of HPA, and HPA in Nanjing Han population has its own characteristics. The highest heterozygotes are HPA-3 and HPA-15, thus more attention to HPA effects on clinical platelet matched transfusion should be paid.