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Updated: May 17, 2026

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Published on: June 3, 2012
Unstable polymerase-nucleoprotein interaction is not responsible for avian influenza virus polymerase restriction in
Anna V Cauldwell1, Olivier Moncorgé, Wendy S Barclay
1Imperial College London, Faculty of Medicine, Division of Infectious Disease, London, United Kingdom.
Abstract:
Avian-origin influenza virus polymerase activity can be dramatically increased in human cells with the PB2 E627K mutation. Previously, others have proposed that this mutation increases the stability of the viral ribonucleoprotein complex (vRNP) measured by the interaction between PB2 and NP. However, we demonstrate here that a variety of PB2 adaptive mutations, including E627K, do not enhance the stability of the vRNP but rather increase the amount of replicated RNA that results in more PB2-NP coprecipitation.
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