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Decreased mitochondrial oxidative phosphorylation capacity in the human heart with left ventricular systolic

Nis Stride1, Steen Larsen, Martin Hey-Mogensen

  • 1Xlab, Center for Healthy Aging, Department of Biomedical Sciences, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3b, DK-2200 Copenhagen, Denmark. nis.stride@gmail.com

European Journal of Heart Failure
|November 2, 2012
PubMed

Insights

Heart failure with left ventricular systolic dysfunction (LVSD) is linked to reduced mitochondrial oxidative phosphorylation (OXPHOS) capacity, especially in fatty acid metabolism. This suggests mitochondrial dysfunction contributes to the energetic deficit in heart failure.

Area of Science:

  • Cardiology
  • Mitochondrial Biology
  • Biochemistry

Background:

  • Heart failure (HF) with left ventricular systolic dysfunction (LVSD) involves altered substrate utilization and energy production.
  • The role of mitochondrial dysfunction in the cardiac phenotype of LVSD remains unclear.

Purpose of the Study:

  • To investigate mitochondrial oxidative phosphorylation (OXPHOS) capacity in LVSD myocardium.
  • To compare OXPHOS capacity in patients with and without LVSD.

Main Methods:

  • Myocardial biopsies from LVSD (LVEF <45%) and control (LVEF >45%) patients.
  • Measurement of mitochondrial respiration using non-fatty acid substrates and medium-chain fatty acid (MCFA).
  • Assessment of mitochondrial creatine kinase (miCK) capacity.

Main Results:

  • Maximal OXPHOS capacity with non-fatty acid substrates was significantly lower in LVSD patients.
  • MCFA oxidation elicited approximately 40% lower respiration in LVSD compared to controls.
  • ADP sensitivity for miCK was highest in the LVSD group.

Conclusions:

  • Human LVSD exhibits significantly diminished OXPHOS capacity, particularly in MCFA oxidation.
  • This finding provides a potential mechanism for the compromised energetic state observed in HF.
  • Reduced reliance on fatty acid utilization may be a hallmark of HF with LVSD.
Abstract

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