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Updated: May 17, 2026

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Published on: April 30, 2014
Is there a role for adult non-cultivated bone marrow stem cells in ACL reconstruction?
Alcindo Silva1, Ricardo Sampaio, Rui Fernandes
1Orthopedics Department, Hospital Militar D. Pedro V, Avenida da Boavista, 4050-113, Porto, Portugal, alcindocsilva@gmail.com.
Purpose:
To assess by magnetic resonance imaging (MRI) if adult non-cultivated bone marrow stem cells accelerate tendon-to-bone healing in the femoral tunnel, after hamstring anterior cruciate ligament (ACL) reconstruction.
Methods:
Forty-three patients underwent ACL reconstruction and were prospectively randomized into two groups: 20 patients in the experimental group (group A) with adult non-cultivated bone marrow stem cells and 23 patients in the control group (group B) without adult non-cultivated bone marrow stem cells. All patients underwent MRI of the knee at three months after surgery to evaluate the signal-to-noise ratio of the interzone.
Results:
There was no difference in the signal-to-noise ratio of the interzone on MRI between the experimental and the control group.
Conclusions:
Adult non-cultivated bone marrow stem cells do not seem to accelerate graft-to-bone healing in ACL reconstruction. The clinical relevance of this finding is that adult non-cultivated bone marrow stem cells apparently have a limited role in ACL reconstruction.
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