Related Experiment Video
Updated: May 17, 2026
The Establishment of Calvarial Suture-Bony Composite Defects in Rats: A Standardized Model for Suture-Regenerative Therapy Investigation
Published on: May 10, 2024
Is a 5 mm rat calvarium defect really critical?
Gabriela Granja Porto1, Belmiro Cavalcanti do Egito Vasconcelos, Emanuel Sávio de Souza Andrade
1Department of Oral and Maxillofacial Surgery, UPE, Recife-PE, Brazil.
Purpose:
To evaluate bone regeneration in critical defects in the rats' calvarium.
Methods:
Eighteen rats Wistar were divided into three groups of six animals each according to the time of evaluation (15, 30 and 60 days). One calvarium defect of 5 mm was made in the parietal bone of each animal under general anesthesia. After the time of evaluation, the animals were killed, when the bone was histological studied and classified according to the type of tissue found: fibrosis or bone.
Results:
The results showed that in the group of 15 days, in five animals there was only fibrosis. In the group of 30 days, the process of regeneration was growing and in four animals was found bone, in three with partial filling and in the other one with complete filling of the defect. In the group of 60 days, out of the three animals with bone, two had a complete filling of the defect.
Conclusions:
There was no bone regeneration in critical defects in 15 days. There was regeneration in the most part of the animals in 30 and 60 days.
