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Protein chemical analysis of purified murine lamin B identifies two distinct polypeptides B1 and B2
K Weber1, U Plessmann, P Traub
1Max Planck Institute for Biophysical Chemistry, Department of Biochemistry, Goettingen, FRG.
Abstract:
Lamin B purified from murine EAT cells was characterized by partial protein sequences. Contrary to the current view that mammals express only a single lamin B polypeptide corresponding to a characterized murine cDNA clone, our analysis documents two distinct B lamins. One protein follows the established cDNA sequence while the other identifies a novel murine lamin B. Comparison with the two chicken lamin B sequences established by cDNA cloning identifies the first murine lamin B sequence as a B1 type and the second as a B2 type. We conclude that mammals express two distinct lamin B forms as established by others for chicken.