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Related Experiment Video

Updated: May 17, 2026

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Published on: July 17, 2020

PNUTS functions as a proto-oncogene by sequestering PTEN.

Sridhar Kavela¹, Swapnil R Shinde, Raman Ratheesh

¹Laboratory of Cell Death & Cell Survival, Centre for DNA Fingerprinting and Diagnostics (CDFD), Nampally, India.

Cancer Research

|November 3, 2012

Summary

Protein phosphatase-1 nuclear targeting subunit PNUTS regulates the tumor suppressor PTEN. PNUTS depletion increases apoptosis and reduces proliferation, suggesting PNUTS is an oncogene in cancer.

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Published on: August 25, 2021

Area of Science:

Molecular biology
Cancer research
Cellular signaling

Background:

PTEN is a crucial tumor suppressor gene regulating the PI3K/Akt pathway.
PTEN controls cell survival, growth, motility, invasiveness, and angiogenesis.
Regulation of PTEN activity in normal and disease states is not fully understood.

Purpose of the Study:

To identify novel regulators of PTEN.
To investigate the role of PNUTS (PPP1R10) in PTEN regulation.
To explore the potential oncogenic role of PNUTS in cancer.

Main Methods:

Protein-protein interaction assays to identify PTEN-associated proteins.
Immunofluorescence and co-immunoprecipitation to study PTEN-PNUTS interaction.
Cellular assays (apoptosis, proliferation) upon PNUTS depletion.
Analysis of PNUTS expression in cancer tissues.

Main Results:

PNUTS (PPP1R10) was identified as a PTEN-associated protein.
PNUTS directly binds to PTEN's C2 domain and sequesters PTEN in the nucleus.
Depletion of PNUTS results in PTEN-dependent apoptosis and reduced proliferation.
PNUTS expression is elevated in certain cancers compared to normal tissues.

Conclusions:

PNUTS is a novel regulator of the tumor suppressor PTEN.
PNUTS interaction with PTEN influences PTEN's cellular localization and function.
PNUTS acts as a PTEN-dependent oncogene, with elevated expression in cancers.