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Interleukin-2 receptor expression in human mast cells and basophils
N Maggiano1, F Colotta, F Castellino
1Istituti di Anatomia Patologica, Università Cattolica S. Cuore, Rome, Italy.
International Archives of Allergy and Applied Immunology
|January 1, 1990
Summary
Human mast cells and basophils express the interleukin 2 receptor (IL2-R) on their surface and actively synthesize it. This finding suggests a role for IL2-R in mast cell and basophil function.
Area of Science:
- Immunology
- Cell Biology
- Histopathology
Background:
- Interleukin 2 receptor (IL2-R) is crucial for T cell activation.
- Mast cells and basophils are key immune cells involved in inflammatory responses.
Purpose of the Study:
- To investigate the presence and expression of IL2-R in human mast cells and basophils.
- To determine if IL2-R is actively synthesized by these cells.
Main Methods:
- Histochemical and immunologic staining of human tissue specimens.
- Ultrastructural analysis using immunogold labeling.
- Northern blot analysis for IL2-R mRNA detection.
Main Results:
- IL2-R positive cells were identified in various human tissues, including those with and without inflammation.
- Mast cells showed distinct IL2-R positivity.
- Peripheral blood basophils (PBB) expressed IL2-R on their surface and intracellularly, indicating active synthesis.
- IL2-R mRNA was detected in PBB, and its expression increased with T cell supernatant stimulation.
Conclusions:
- Human mast cells express the p55 chain of IL2-R.
- Peripheral blood basophils actively synthesize IL2-R.
- These findings suggest a potential role for IL2-R in mast cell and basophil biology and function.