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MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
Host factors with regulatory roles in tombusvirus replication
Peter D Nagy1, Daniel Barajas, Judit Pogany
1Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA. pdnagy2@uky.edu
Current Opinion in Virology
|November 6, 2012
Summary
Plant positive-strand RNA viruses, like tombusviruses, hijack host resources for replication. The tombusvirus p33 protein interacts with host factors, organizing viral replication through specific task commitment.
Area of Science:
- Molecular Virology
- Plant Pathology
- Host-Virus Interactions
Background:
- Plant positive-strand RNA viruses utilize host cell machinery for replication.
- Tombusviruses are a significant group of plant RNA viruses.
- Understanding virus-host interactions is crucial for controlling plant viral diseases.
Purpose of the Study:
- To review virus-host interactions during tombusvirus replication.
- To elucidate the multifunctional role of the tombusvirus p33 replication protein.
- To explain the mechanism of p33's diverse functions in viral replication.
Main Methods:
- Review of existing literature on tombusvirus replication and host interactions.
- Analysis of protein-protein interactions involving the tombusvirus p33 protein.
- Examination of the role of host factors in regulating viral replication.
Main Results:
- The tombusvirus p33 protein interacts with itself, viral components (p92 polymerase, RNA), and approximately 100 host proteins and membranes.
- Negative regulatory host proteins (e.g., cyclophilins, WW motif proteins) bind to p33 and inhibit its functions.
- A model is proposed where p33 interactions commit it to specific tasks, organizing replication spatially and temporally.
Conclusions:
- The tombusvirus p33 protein is a central hub orchestrating viral replication through extensive host interactions.
- Specific binding events involving p33 dictate its function and localization within the infected cell.
- This interaction-driven compartmentalization of p33 is key to efficient tombusvirus replication.
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