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Updated: May 17, 2026

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

Published on: October 1, 2014

Capturing single L-type Ca(2+) channel function with optics.

Matthew A Nystoriak¹, Madeline Nieves-Cintrón, Manuel F Navedo

¹Department of Pharmacology, University of California Davis, Davis, CA, USA.

Biochimica Et Biophysica Acta

|November 6, 2012

Summary

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Optical imaging reveals new insights into L-type voltage-dependent calcium channels (LTCCs). Single LTCCs ("sparklets") show heterogeneous activity and cooperative gating, informing models of channel control.

Area of Science:

Biophysics
Cellular Physiology
Molecular Biology

Background:

Optical imaging advances enable detailed analysis of Ca(2+)-permeable ion channel function.
L-type voltage-dependent calcium channels (LTCCs) play critical roles in cellular signaling.

Purpose of the Study:

To review novel optical recording methods for LTCC function.
To discuss new functional characteristics of LTCCs revealed by advanced imaging.

Main Methods:

Total internal reflection fluorescence (TIRF) microscopy for high-resolution optical measurement.
Visualization of Ca(2+) influx through single LTCCs (
LTCC sparklets
)

Main Results:

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Last Updated: May 17, 2026

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

Published on: October 1, 2014

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Imaging Ca²⁺ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Published on: May 6, 2015

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca²⁺ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

LTCC activity is regionally heterogeneous.
Clustered LTCCs exhibit cooperative or coupled gating.
Single channel activity (
LTCC sparklets
) provides insights into channel function.

Conclusions:

A molecular model for local control of LTCC activity and coupled gating is proposed.
Findings have implications for understanding LTCCs in physiological and pathological conditions.