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Updated: May 17, 2026
05:47
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Scanning probe electrospray ionization for ambient mass spectrometry.
Yoichi Otsuka1, Sayuri Shide, Junpei Naito
1Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo, 146-8501, Japan. otsuka.yoichi@canon.co.jp
Rapid Communications in Mass Spectrometry : RCM
|November 6, 2012
Summary
A novel scanning probe electrospray ionization (SPESI) method enables rapid, direct sampling and ionization for mass spectrometry. This technique utilizes a vibrating capillary probe for efficient analysis of solid samples with minimal cross-contamination.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Surface Science
Background:
- Ambient sampling and ionization techniques are crucial for rapid mass spectrometry analysis.
- Existing methods often require complex sample preparation or lack direct sampling capabilities.
Purpose of the Study:
- To develop a novel method for direct, local sampling and ionization using a single vibrating capillary probe.
- To enable rapid analysis in mass spectrometry with enhanced spatial resolution.
Main Methods:
- A vibrating capillary probe supplies charged solvents to form a liquid bridge for sampling and electrospray ionization (ESI).
- Two modes were employed: contact-mode (sampling and ionization co-existed) and tapping-mode (sampling and ionization were separated).
- Ions were detected using a triple quadrupole mass spectrometer.
Main Results:
- The scanning probe electrospray ionization (SPESI) method successfully achieved simultaneous sampling and ionization at the probe apex.
- One-dimensional mapping of solid samples, including protein films and tissue sections, was demonstrated.
- The method showed minimal cross-contamination, preserving sample integrity.
Conclusions:
- SPESI offers a simple and unique approach for direct sampling and ionization in mass spectrometry.
- The technique holds promise for rapid, high-resolution analysis of various solid samples.
- This method advances ambient ionization techniques for imaging mass spectrometry.
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