Related Experiment Video
Updated: May 17, 2026
Establishment of an Embryo Implantation Model In Vitro
Published on: June 21, 2024
[In vitro embryo culture of Epimedium wushanense]
Haiqin Zhou1, Guosheng Zhiu, Qiaosheng Guo
1Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China.
Objective:
To study the in vitro embryo culture of Epimedium wushanense and provide scientific basis for large scale production of tissue culture.
Method:
Cullus and buds were induced from embryo of E. wushanense on a MS medium supplemented with different 2,4-D,6-BA, NAA, IBA.
Result:
The optimal compositions of medium that induced callus and buds from embryo were the MS medium supplemented with 2,4-D 2 mg x L(-1), IBA 2 mg x L(-1) and NAA 0.5 mg x L(-1) and the MS medium supplemented with IBA 2 mg x L(-1) and 6-BA 0.5 mg x L(-1), respectively. The optimum medium for callus differentiation was MS + 6-BA 1 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 1 mg x L(-1), and MS +6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) for shoots proliferation.
Conclusion:
Using embryo as explants, the method of induction and culture of E. wushanense was established by the callus and buds, and the embryo of E. wushanense can be quickly propagated.
