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Purification and amino-terminal sequence analysis of the complement-fixing and precipitin antigens from Coccidioides
S Resnick1, B Zimmer, D Pappagianis
1Department of Dermatology, University of California, San Francisco 94143.
Journal of Clinical Microbiology
|February 1, 1990
Abstract:
Two proteins (21 and 48 kilodaltons) purified from endospore-spherule culture filtrates of Coccidioides immitis are identified as precipitin and complement-fixing antigens, respectively. To allow specific structural comparison to antigens identified by other investigators and as a first step to eventual serodiagnostic antigen production by recombinant DNA technology, amino-terminal amino acid sequences were determined for these antigens.