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Updated: May 17, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B
C J Kasanga1, T Yamaguchi, H M Munang'andu
1Department of Veterinary Microbiology and Parasitology, Molecular Biology and Biotechnology Section, Sokoine University of Agriculture, P. O. Box 3019, Chuo kikuu, Morogoro, Tanzania. chrisskasa@gmail.com
Abstract:
We determined the complete nucleotide sequence of an infectious bursal disease (IBD) virus (IBDV) isolate (designated KZC-104) from a confirmed IBD outbreak in Lusaka in 2004. The genome consisted of 3,074 and 2,651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent (VV) strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segment A and B sequences showed 98 % nucleotide sequence identity to the VV strain D6948 and 99.8 % nucleotide sequence identity to the classical attenuated strain D78. This is a unique IBDV reassortant strain that has emerged in nature, involving segment B of a cell-culture-adapted attenuated vaccine.
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