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Synthesis and fluorescence study of a quaternized copolymer containing pyrene for DNA-hybridization detection

Lingye Yang1, Min Zhao, Ruichen Zhang

  • 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|November 7, 2012
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...