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A simple method for isolation of DNA fragments associated with the nuclear lamina in vivo
R Christova1, Z Galcheva-Gargova
1Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Abstract:
We describe a simple method for the purification of DNA fragments associated with the nuclear lamina in vivo. Ehrlich ascite tumor cells are first u.v.-irradiated to crosslink DNA to proteins. The nuclear lamina is then isolated and purified by low-speed centrifugation through a cushion of 40% sucrose. The material sedimenting through the created density barrier represents nuclear lamina of a very high purity, free from any DNA fragments except those which were in a crosslinking distance to it in vivo.
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