Related Experiment Video
Updated: May 17, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Preparation and evaluation of C10-cationic latex particle coated open-tubular column for capillary
Xuefang Diao1, Feifang Zhang, Bingcheng Yang
1School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
A simple way was proposed to prepare reverse phase open tubular column (OTC) for capillary electrochromatography (CEC) by dynamically coating cationic latex particles with C(10) functional groups onto the fused silica capillary with surface sulphonation. The latex particles are bounded into the capillary wall via electrostatic attraction force and demonstrate good stability under test conditions. Relative bare silica capillary, reverse electroosmotic flow (EOF) is generated for the OTC due to the existence of cationic latex particles onto the capillary. Preliminary separation of aromatic compounds on an OTC with 50 i.d. has been demonstrated.
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Capillary Electrophoresis: Instrumentation
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
