Related Experiment Videos
A facile synthesis of [14C] epicholesterol
1Department of Chemistry and Biochemistry, Queens College, City University of New York, Flushing 11367.
Journal of Lipid Research
|January 1, 1990
Abstract:
A facile procedure is described for the preparation of [14C]epicholesterol from [14C]cholesterol. Cholesterol is first converted to cholesteryl mesylate, which is treated with cesium acetate and 18-crown-6 in refluxing toluene to give epicholesteryl acetate. The latter is hydrolyzed, without isolation, with potassium hydroxide in tetrahydrofuran-methanol to give epicholesterol, which is obtained in pure form by preparative thin-layer chromatography.