Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Carnosine Supplementation on Warmed-Over Flavor, Volatile Compounds, and Meat Quality in Ground Chicken Breast and Thigh Meat.

The journal of poultry science·2026
Same author

Japanese-Style Diet and LOX-Index in Japanese Municipal Workers.

Journal of nutritional science and vitaminology·2026
Same author

Infliximab as an Effective Second-Line Treatment for IVIG-Resistant Kawasaki Disease Accompanied by Severe Dyslipidemia.

Pediatric cardiology·2026
Same author

Biphasic dextromethorphan O-demethylation in tree shrews mediated by liver microsomal cytochromes P450 with high and low affinities.

Drug metabolism and disposition: the biological fate of chemicals·2026
Same author

The Added Value of Systematic Biopsy in Biopsy-Naïve Patients Undergoing Magnetic Resonance Imaging-Transrectal Ultrasound Fusion Prostate Biopsy.

Research and reports in urology·2026
Same author

Structure-Dynamics-Property Relationships in Silk Fibroin Films Probed via Solid-State NMR.

ACS omega·2026

Related Experiment Video

Updated: May 17, 2026

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

Rat stem-cell leukemia gene expression increased during testis maturation.

Ahmed Magzoub Khalid1, Atsushi Asano, Yoshinao Z Hosaka

  • 1Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.

Bioscience, Biotechnology, and Biochemistry
|November 8, 2012
PubMed
Summary

The stem-cell leukemia (SCL) gene, Tal-1, is upregulated in maturing rat testes, suggesting a role in spermatogenesis. Tal-1 may also be a novel cancer-testis antigen candidate.

More Related Videos

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Germ Cell Transplantation and Testis Tissue Xenografting in Mice
10:41

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

Related Experiment Videos

Last Updated: May 17, 2026

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Germ Cell Transplantation and Testis Tissue Xenografting in Mice
10:41

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Oncology

Background:

  • Stem-cell leukemia (SCL), also known as Tal-1, is a critical gene in hematopoiesis.
  • Its role in testicular development and cancer-testis antigenicity is not fully understood.

Purpose of the Study:

  • To investigate the expression and potential role of Tal-1 in the maturing rat testis.
  • To explore Tal-1 as a potential cancer-testis antigen.

Main Methods:

  • Northern blotting to detect Tal-1 gene expression.
  • Western blotting to confirm protein size.
  • Immunohistochemistry to analyze protein localization in testicular tissues.
  • Analysis of NKX3.1 gene expression correlation.

Main Results:

  • Tal-1 gene expression was significantly upregulated in the maturing rat testis.
  • Tal-1 protein (approx. 34 kDa) was detected in spermatocytes, spermatids, and spermatogonia.
  • Tal-1 expression showed a negative correlation with the regulatory gene NKX3.1.
  • Human Tal-1 expression was observed in both maturing testis and bone marrow.

Conclusions:

  • Tal-1 is involved in cell division, morphological changes, and spermatogenic cell development in the rat testis.
  • Tal-1 is a potential novel cancer-testis antigen candidate due to its expression in both testis and bone marrow.