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Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

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Replication in Eukaryotes02:31

Replication in Eukaryotes

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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Relationship between DNA replication and the nuclear matrix.

Rosemary H C Wilson1, Dawn Coverley

  • 1Department of Biology, University of York, Heslington, York, YO10 5DD, UK.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|November 9, 2012
PubMed
Summary
This summary is machine-generated.

The nuclear matrix (NM) immobilizes DNA replication, including both initiation and elongation. This review highlights NM-DNA interactions and their role in DNA synthesis across different cell types.

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Published on: January 19, 2017

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The nuclear matrix (NM) is an internal structure within the cell nucleus.
  • Its role in DNA replication has been investigated, but challenges exist in studying this complex structure.
  • Differences in NM function may arise depending on the model system used.

Purpose of the Study:

  • To review the relationship between the nuclear matrix and DNA replication.
  • To highlight difficulties in studying the nuclear matrix and potential model system variations.
  • To discuss DNA attachment regions, their characteristics, and interactions with replication factors.

Main Methods:

  • Literature review of primary published work on the nuclear matrix and DNA replication.
  • Analysis of data concerning DNA synthesis, nuclear matrix association, and replication proteins.
  • Examination of evidence for transient recruitment of replication machinery to the NM.

Main Results:

  • Newly synthesized DNA is associated with a nuclease-resistant nuclear matrix, indicating immobilized DNA elongation.
  • Prereplication complex proteins and origins of replication are transiently recruited to the NM during late G1 and early S-phase.
  • Both initiation and elongation steps of DNA replication appear to be immobilized by attachment to the NM.

Conclusions:

  • The nuclear matrix plays a crucial role in organizing and immobilizing DNA replication within the nucleus.
  • Emerging evidence suggests cell-type specific differences in nuclear matrix involvement in DNA replication.
  • Further research is needed to fully elucidate the spatial relationships and functional significance of NM-DNA interactions in replication.