Related Experiment Video
Updated: May 17, 2026
Surgical Bone Implantation Technique for Rat Tibia Models of Diabetes and Osteoporosis
Published on: July 5, 2024
[Differential expression genes of bone tissues surrounding implants in diabetic rats by gene chip]
Xin-xin Wang1, Yue Ma, Qing Li
1Shandong Provincial Key Laboratory of Oral Biomedicine, Shandong Province, China. xinxin1986815918@yahoo.com.cn
Purpose:
To compare mRNA expression profiles of bone tissues surrounding implants between normal rats and rats with diabetes using microarray technology.
Methods:
Six Wistar rats were randomly selected and divided into normal model group and diabetic group. Diabetic model condition was established by injecting Streptozotocin into peritoneal space. Titanium implants were implanted into the epiphyseal end of the rats' tibia. Bone tissues surrounding implant were harvested and sampled after 3 months to perform comprehensive RNA gene expression profiling, including 17983 for genome-wide association study.GO analysis was used to compare different gene expression and real-time PCR was used to confirm the results on core samples.
Results:
The results indicated that there were 1084 differential gene expression. In the diabetic model, there were 352 enhanced expression genes, 732 suppressed expression genes. GO analysis involved 1154 different functional type.
Conclusions:
Osteoblast related gene expressions in bone tissue samples of diabetic rats were decreased, and lipid metabolism pathway related gene expression was increased.
