Related Experiment Video

Updated: May 17, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism
07:36

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

[Device and programs for investigation of biological objects autofluorescence].

A A Klimov, D A Klimov

    Biofizika
    |November 10, 2012
    PubMed
    Summary

    This study introduces a novel fluorescent microscopy method using activatable dyes for super-resolution imaging. It achieves nanoscale precision by repeatedly activating, imaging, and bleaching single molecules to reconstruct detailed object structures.

    More Related Videos

    Autofluorescence Imaging to Evaluate Red Algae Physiology05:54

    Autofluorescence Imaging to Evaluate Red Algae Physiology

    Published on: February 17, 2023

    Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation15:27

    Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

    Published on: February 8, 2015

    Related Concept Videos

    Immunofluorescence Microscopy01:12

    Immunofluorescence Microscopy

    A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
    The...
    Fluorescence and Phosphorescence: Instrumentation01:25

    Fluorescence and Phosphorescence: Instrumentation

    Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.