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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: May 17, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Recent advances in nanopore sequencing.

Raj D Maitra1, Jungsuk Kim, William B Dunbar

  • 1Department of Computer Engineering, Baskin School of Engineering, University of California, Santa Cruz, CA 95064, USA.

Electrophoresis
|November 10, 2012
PubMed
Summary
This summary is machine-generated.

Nanopore sequencing offers a promising new method for DNA analysis, with commercial devices emerging. Scalability of nanopore circuitry is key for future success in next-generation sequencing.

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Last Updated: May 17, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

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Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

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10:34

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Published on: March 15, 2019

Area of Science:

  • Biotechnology
  • Genomics
  • Molecular Biology

Background:

  • Nanopore sequencing has been a focus of significant research for over a decade.
  • Commercialization of nanopore sequencing is imminent with devices announced by Oxford Nanopore Technologies.
  • Several companies are actively developing nanopore sequencing technologies.

Purpose of the Study:

  • To review the current state of nanopore sequencing technology.
  • To highlight recent advancements with commercial potential in next-generation sequencing.
  • To assess the scalability of nanopore circuitry for future devices.

Main Methods:

  • Review of recent scientific literature and commercial announcements.
  • Analysis of technological contributions to nanopore sequencing.
  • Evaluation of scalability factors for multichannel nanopore arrays.

Main Results:

  • Nanopore sequencing is nearing commercial viability.
  • Recent contributions show promise for next-generation sequencing applications.
  • Circuitry scalability is a critical factor for widespread adoption.

Conclusions:

  • Nanopore sequencing represents a significant advancement in DNA sequencing technology.
  • The commercial potential of nanopore sequencing is substantial.
  • Continued research into scalable circuitry is essential for realizing the full capabilities of nanopore sequencing.