Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

Introduction to Nuclear Reprogramming

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Protocol for somatic cell nuclear transfer in the common marmoset.

STAR protocols·2026

Same author

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026

Same author

Distinct allergen-specific T cell receptor clonotypes drive divergent immune programs in a cloned mouse model of atopic dermatitis.

Allergology international : official journal of the Japanese Society of Allergology·2026

Same author

Mouse ICSI impairs fertility of male offspring by disruption of fetomaternal tolerance.

Development (Cambridge, England)·2026

Same author

Messenger RNA delivery into Sertoli cells restores fertility to congenitally infertile male mice.

Stem cell reports·2026

Same author

Generation of functionally competent testicular somatic cells from pluripotent stem cells.

Science advances·2026

Search research articles

Related Experiment Video

Updated: May 17, 2026

Nuclear Transfer into Mouse Oocytes

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

Nuclear transfer in the mouse oocyte.

Eiji Mizutani¹, Atsuo Ogura, Teruhiko Wakayama

¹Center for Developmental Biology, RIKEN Kobe institute, Kobe, Japan, mizutani@rtc.riken.jp.

Methods in Molecular Biology (Clifton, N.J.)

|November 10, 2012

Summary

This summary is machine-generated.

Nuclear transfer (NT) in mice allows cloning and creating embryonic stem cells. This technique bypasses traditional methods, enabling genetic material preservation from living or dead cells.

More Related Videos

Capturing Cytoskeleton-Based Agitation of the Mouse Oocyte Nucleus Across Spatial Scales

Capturing Cytoskeleton-Based Agitation of the Mouse Oocyte Nucleus Across Spatial Scales

Published on: January 12, 2024

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

Related Experiment Videos

Last Updated: May 17, 2026

Nuclear Transfer into Mouse Oocytes

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

Capturing Cytoskeleton-Based Agitation of the Mouse Oocyte Nucleus Across Spatial Scales

Capturing Cytoskeleton-Based Agitation of the Mouse Oocyte Nucleus Across Spatial Scales

Published on: January 12, 2024

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

Area of Science:

Reproductive Biology
Developmental Biology
Genetics

Background:

Nuclear transfer (NT) is a key technique for cloning and deriving embryonic stem cells.
Traditional NT methods often involve cell fusion, which can be complex and time-consuming.

Purpose of the Study:

To describe optimized protocols for mouse nuclear transfer.
To detail the generation of cloned mice and NT-derived embryonic stem (ntES) cells.
To highlight the advantages of direct nuclear injection over cell fusion methods.

Main Methods:

Direct nuclear injection into mouse oocytes using a piezo impact drive.
Application of NT protocols for cloning live mice.
Establishment of ntES cells from cloned embryos.

Main Results:

Successful generation of cloned mice through direct nuclear injection.
Establishment of ntES cells from cloned embryos.
Demonstration that NT can reveal epigenetic modifications without genetic manipulation.

Conclusions:

Direct nuclear injection is an efficient method for mouse cloning and ntES cell generation.
NT techniques offer a valuable approach for preserving genetic material from various sources, including dead cells.
This method bypasses several steps of conventional cell fusion procedures, simplifying the NT process.