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Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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Related Experiment Video

Updated: May 17, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Long non-coding RNAs in stem cell pluripotency.

Shi-Yan Ng1, Lawrence W Stanton

  • 1Genome Institute of Singapore, Stem Cell and Developmental Biology Group, Singapore.

Wiley Interdisciplinary Reviews. RNA
|November 10, 2012
PubMed
Summary
This summary is machine-generated.

Long non-coding RNAs (lncRNAs) are key regulators in maintaining embryonic stem cell (ESC) pluripotency. This review explores how these crucial molecules integrate into the complex gene regulatory networks governing stem cell self-renewal.

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Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
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Related Experiment Videos

Last Updated: May 17, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
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Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

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CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

Area of Science:

  • Stem cell biology
  • Molecular genetics
  • Epigenetics

Background:

  • Pluripotency in embryonic stem cells (ESCs) is maintained by complex gene regulatory networks.
  • These networks involve transcription factors, genomic targets, and epigenetic modifications.
  • Long non-coding RNAs (lncRNAs) are emerging as critical components of these networks.

Purpose of the Study:

  • To discuss the integration of lncRNAs into the pluripotency gene regulatory network.
  • To highlight the role of lncRNAs in maintaining and inducing stem cell pluripotency.
  • To identify prominent questions in the emerging field of pluripotent lncRNAs.

Main Methods:

  • Literature review of recent reports on pluripotent lncRNAs.
  • Analysis of lncRNA mechanisms in gene regulation.
  • Discussion of lncRNA integration into pluripotency networks.

Main Results:

  • lncRNAs are defined as RNA transcripts >200 nucleotides with limited protein-coding potential.
  • Pluripotent lncRNAs have been identified that are involved in stem cell pluripotency maintenance and induction.
  • lncRNAs act via diverse mechanisms to influence cellular functions.

Conclusions:

  • lncRNAs are integral to the gene regulatory networks governing ESC pluripotency.
  • Further research is needed to fully understand the mechanisms and roles of lncRNAs in stem cell biology.
  • The field of pluripotent lncRNAs presents exciting avenues for future investigation.