Related Experiment Video
Updated: May 17, 2026

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
Published on: October 20, 2011
In vitro evaluation of microbiological flora of orofacial infections
Munish Kohli1, Asha Mathur, Monica Kohli
1Dept. of Oral and Maxillofacial Surgery, Saraswathi Dental College and Hospital, Lucknow, India ; 26, Napier Road Colony Part 1 Thakurganj, Lucknow Uttarpradesh, India.
Objective:
To assess the most common micro-organisms causing odontogenic infections and their antimicrobial susceptibility.
Methods:
The study was conducted in 80 patients with orofacial infection. The pus sample was collected, cultured (aerobically and anaerobically) and stained for morphological study of the isolates. Antibiotic sensitivity test for the isolates were performed.
Results:
A total of 109 micro-organisms were isolated, no pathogenic organism were isolated in 3 cases. Out of 109 micro-organism isolated, 107 bacteria and 2 fungi were identified. Pure aerobes were identified in 28(35%) of cases, pure anaerobes in 18(22.5%), mixed aerobes and anaerobes in 10(12.5%), mixed aerobes in 15(18.75%) and mixed anaerobes were isolated in 6(7.5%) cases. Among the entire pure gram positive isolates, ofloxacin was the most sensitive drug 83.33% followed by ciprofloxacin 76.2% and sparfloxacin 76.2%. The most resistant drugs were amoxicillin (92.85%) and ampicillin (92.85%). Cefotaxime was found sensitive in 75% of pure gram negative isolates.
Conclusion:
Ofloxacin was the most sensitive drug followed by ciprofloxacin and sparfloxacin for pure gram positive isolates. The most resistant drugs were amoxicillin and ampicillin. The gram negative colonies were sensitive to Cefotaxime.
Related Concept Videos
The Oral Microbiota
Development of the Oral Microbiota
Assessment of the Mouth
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.
Methods to Assess Microbial Populations

