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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: May 17, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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MicroRNAs in human lymphoblastoid cell lines.

Sung-Mi Shim1, Hye-Young Nam, Jae-Eun Lee

  • 1National Biobank of Korea, Center for Genome Science, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, 200 Osongsaengmyung-2-ro, Osong-eup, Chungwon-gun, Chungbuk-do, 363-951, South Korea.

Critical Reviews in Eukaryotic Gene Expression
|November 13, 2012
PubMed
Summary

Human lymphoblastoid cell lines (LCLs) are valuable for genetic and immunological research. These cell lines help study microRNA-mediated gene expression and its role in diseases and transcriptional regulation.

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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Area of Science:

  • Genomics
  • Epigenetics
  • Cell Biology

Background:

  • Human lymphoblastoid cell lines (LCLs) are derived from EBV-transformed B cells, offering a renewable source for genetic and immunological studies.
  • LCLs serve as an in vitro model to analyze population variations in gene and microRNA expression and cellular responses.
  • Their utility extends to pharmacogenomics for identifying genetic factors in individual responses to environmental factors.

Purpose of the Study:

  • To explore the role of microRNA-mediated gene expression in LCLs.
  • To discuss the application of LCLs in disease genomics and the study of transcriptional regulatory networks.
  • To highlight the importance of epigenetic regulation by microRNA and DNA methylation in disease pathogenesis and therapeutic target discovery.

Main Methods:

  • Utilizing LCLs for generating cellular phenotypes and diverse genomic data (SNP, CNV, transcriptome, methylome).
  • Linking genomic data from LCLs to donor clinical information for integrative analyses.
  • Reviewing existing literature on miRNA-mediated gene expression and its applications.

Main Results:

  • Genomic signatures in LCLs can differentiate patients with brain diseases and non-lymphoid tumors from controls.
  • MicroRNA expression is often induced in abnormal cellular conditions like viral infections or cancer.
  • Epigenetic regulation via miRNA and DNA methylation is crucial for understanding complex diseases and cancer.

Conclusions:

  • LCLs are versatile tools for integrative genomic analyses, linking cellular phenotypes to donor data.
  • MicroRNA-mediated gene expression in LCLs has significant implications for disease genomics.
  • Understanding these mechanisms is vital for discovering therapeutic targets and advancing disease research.