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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Feb 28, 2026

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Published on: April 9, 2017

Fake virus particles generated by fluorescence microscopy.

Patrick Forterre¹, Nicolas Soler, Mart Krupovic

¹Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS UMR 8621, 91405 Orsay Cedex, France. patrick.forterre@igmors.u-psud.fr

Trends in Microbiology

|November 13, 2012

Summary

This summary is machine-generated.

Fluorescent dyes used in environmental virology can overestimate virus numbers due to interference from other DNA sources. Future studies should improve detection methods and focus on specific virus groups for accurate viral abundance assessments.

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Area of Science:

Environmental virology
Microbial ecology

Background:

Laboratories study viral abundance and roles in ecosystems.
Fluorescent dyes are commonly used to quantify viral particles.

Purpose of the Study:

To highlight potential overestimation of virus numbers in environmental virology studies.
To suggest alternative approaches for accurate viral quantification.

Main Methods:

Analysis of fluorescence-based viral detection methods.
Identification of sources of false positives in viral counts.

Main Results:

Fluorescent dots from membrane-derived vesicles (MVs), gene transfer agents (GTAs), or cell debris can be mistaken for viral particles.
Current fluorescence counting methods may lead to inaccurate overestimation of virus abundance.

Conclusions:

Environmental virology studies must address the issue of false positives in viral quantification.
Future research should focus on differentiating virions from MVs and adopting targeted approaches for virus detection and isolation.